by Katy | Nov 19, 2021
This stain is an excellent general stain that is useful in a clinical setting as most fungi take up the stain. It is especially good for detecting yeast cells and hyphae in tissues. Fungi are stained a bright pink-magenta or purple against an: orange background of...
by Katy | Nov 19, 2021
An aliquot of specimen is added to a drop of 20% KOH on a glass slide. This technique is used to determine fungi in thick mucoid specimens or in specimens that contain keratinous material such as skin, hair and nails. The protein components of the host cells are...
by Katy | Nov 19, 2021
This technique is used in histology laboratories on fixed and sectioned tissues. Fungal elements take up the stain and appear pink to pinkish blue colour (Fig. 2.5). However, the success of the staining is species depend as other take it up more readily than others....
by Katy | Nov 19, 2021
More commonly used for identification of bacteria. However, fungal cells are larger than bacteria and readily take up the stain (Fig 2.4). The Gram stain is performed routinely to detect bacteria in clinical specimens sent for general microbiology. Most fungi present...
by Katy | Nov 19, 2021
This is used for examination of fixed, deparaffinized tissue, therefore allows you to visualize fungi in tissues. Fungal structures are stained black and easily identified from a pale green or yellow background (Fig 2.3). Figure 2.3 Grocott-Methenamine Silver Stain...
