More commonly used for identification of bacteria. However, fungal cells are larger than bacteria and readily take up the stain (Fig 2.4). The Gram stain is performed routinely to detect bacteria in clinical specimens sent for general microbiology. Most fungi present in a Gram-stained smear are detectable and suspected fungal elements may be easily confirmed by KOH and/or calcofluor white.
Figure 2.4 Gram Stain of Candida krusei. © Mycology Reference Center Manchester 2016.
Table 1.4 Advantages and disadvantages of Gram stain